https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:42468 Wed 24 Aug 2022 11:40:43 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:51162 Wed 23 Aug 2023 17:25:12 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:26761 Wed 11 Apr 2018 15:56:38 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:26732 Wed 11 Apr 2018 15:18:52 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:28205 Wed 11 Apr 2018 15:15:40 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:26763 Wed 11 Apr 2018 15:09:54 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:13613 Wed 11 Apr 2018 14:02:01 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:16614 Wed 11 Apr 2018 12:11:43 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:15206 Wed 11 Apr 2018 11:04:16 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:22643 Wed 11 Apr 2018 10:46:26 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:29979 Wed 04 Sep 2019 10:18:53 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:33387 34-weeks gestation; n=8) and gestational age matched preterm (31.6-35.1 weeks; n=8) and term normotensive controls were also compared. Agilent Human miRNA microarray v19 was used to detect up to 2006 miRNAs in four placentae from each group. Statistically different levels of expression were determined and refined using predictive modelling. Placental miRNAs predicted to target RAS mRNAs were identified in three databases. Differences detected on the array were confirmed for some miRNAs by semi-quantitative RT-PCR (qPCR, n=7-8 for all groups). Two differentially expressed miRNAs that were known to target human renal REN mRNA (miR-181a-5p and miR-663) were transfected into human HTR-8/SVneo trophoblast cells to examine their effect on placental REN expression and prorenin levels. In early gestation placentae, 186 miRNAs were differentially expressed compared with term placentae (109 increased, 77 decreased). Thirty of the differentially expressed miRNAs were predicted to target RAS components. In mid-gestation placentae, 117 miRNAs were differentially expressed compared with term placentae (69 increased, 48 decreased). Of these, 19 had RAS mRNAs as predicted targets. Eight miRNAs that were lower in early gestation and predicted to target RAS mRNAs were confirmed by qPCR. All showed an increase during gestation and could influence the transgestational profile of the human placental RAS. Additionally, on the array, three miRNAs predicted to target RAS mRNAs (miR-892c-3p, miR-378c and miR-514b-3p) were overexpressed in placentae from women with late-onset PE (P = 3.6E-10, P = 1.8E-05, P = 5.3E-06; respectively). miR-663, which suppresses renal REN mRNA expression, was overexpressed in early-onset PE placentae as determined by qRT-PCR analysis (P = 0.014). Transfection of miR-181a-5p and miR-663 into HTR-8/SVneo trophoblast cells suppressed REN mRNA expression (p = 0.05) and prorenin protein production (P = 0.001). Data can be found via GEO accession number GSE109832. Further validation that the differentially expressed miRNAs do indeed directly target RAS mRNAs and affect placental development and function is required. This study is limited by the small sample size. Therefore independent validation in a larger cohort is required.]]> Wed 02 Mar 2022 14:28:37 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:26759 Tue 24 Apr 2018 15:35:25 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45233 Thu 27 Oct 2022 13:10:07 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:26776 Thu 11 Apr 2019 13:18:13 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:37374 1/2) of Gd₂O₃-Co/NG is 100 mV more positive than that of Co/NG and even close to commercial Pt/C. The density functional theory calculation and spectroscopic analysis demonstrate that, owing to intrinsic charge redistribution at the engineered interface of Gd₂O₃/Co, the coupled Gd₂O₃-Co can break the OOH*-OH* scaling relation and result in a good balance of OOH* and OH* binding on Gd₂O₃-Co surface. For practical application, a rechargeable Zn-air battery employing Gd₂O₃-Co/NG as an air-cathode achieves a large power density and excellent charge-discharge cycle stability.]]> Mon 29 Jan 2024 18:04:04 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:46288 Mon 14 Nov 2022 16:16:51 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:36201 Mon 02 Mar 2020 11:21:09 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:51702 Fri 15 Sep 2023 13:41:02 AEST ]]>